منابع مشابه
Direct analysis of mAb aggregates in mammalian cell culture supernatant
BACKGROUND Protein aggregation during monoclonal antibody (mAb) production can occur in upstream and downstream processing (DSP). Current methods to determine aggregate formation during cell culture include size exclusion chromatography (SEC) with a previous affinity chromatography step in order to remove disturbing cell culture components. The pre-purification step itself can already influence...
متن کاملEffects of Lactobacillus casei Culture Supernatant on Differentiation of K562 Cell Line
ABSTRACT Background and objective: Considering the toxic side effects of chemotherapy in treatment of cancer, anticancer drugs of natural origin including probiotic Lactobacillus strains have recently attracted a lot of attention. Methods: After culturing chronic myeloid leukemia cell line K562 in 96-well plates, effects of different concentrations of ...
متن کاملMycobacterium tuberculosis Culture Supernatant Induces Cancer Cell Apoptosis and Cell Cycle Arrest
Human lung cancer remains one of the deadliest diseases worldwide. New approaches are needed for improved lung cancer treatment. In this study, we found that M. tuberculosis culture supernatant (TB-SN) could inhibit human lung cancer cell proliferation through a caspase-dependent apoptosis pathway and induce cell cycle arrest in G1 phase. The active components responsible for the growth inhibit...
متن کاملImproving the performance of a biofuel cell cathode with laccase-containing culture supernatant from Pycnoporus sanguineus.
Laccases are multicopper oxidoreductases that can be used in biofuel cells to improve cathode performance by cathodic oxygen reduction. Here we present a laccase from the ligninolytic white-rot fungus Pycnoporus sanguineus that, in contrast to the Trametes versicolor laccase, can be produced in the absence of inducers in a standard culture medium. After 7days of cultivation the activity of this...
متن کاملOptimized exosome isolation protocol for cell culture supernatant and human plasma
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and prognosis of disease. However, there is currently limited information elucidating the most efficient methods for obtaining high yields of pure exosomes, a subset of extracellular vesicles, from cell culture supernatant and complex biological fluids such as plasma. To this end, we comprehensively characterize...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2015
ISSN: 2043-0116
DOI: 10.1038/protex.2015.107